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. 2014 Mar 7;9(3):e89899. doi: 10.1371/journal.pone.0089899

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© 2014 Konitsiotis et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A. Localization of Hhat in PANC1 cells was assessed using Hhat-EGFP transfection and confocal microscopy combined with immunofluorescence localization of ER (PDI) and Golgi (GM130). B. HEK293a Hhat-V5 stable cells were co-stained for the V5 epitope with ER (Calnexin) or Golgi (GM130) and nuclei (DAPI). The data show that both Hhat-EGFP and Hhat-V5 localize primarily in ER with little if any in Golgi apparatus. Scale bar = 10 µm.