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. 2014 Mar 7;9(3):e90534. doi: 10.1371/journal.pone.0090534

Figure 3. Treatment of NIH-3T3 cells with cyclopamine but not IPI-926 confers hyper-responsiveness to Shh stimulation.

Figure 3

Cells were pre-treated with either cyclopamine or IPI-926 for 24 h followed by extensive washing and subsequent treatment with either media alone or media containing 100 nM rShh. Gli1 mRNA levels were assessed by qRT-PCR 21 h post stimulation. Hyper-responsiveness to rShh stimulation is observed in NIH-3T3 cells treated with high doses of cyclopamine (A) but not IPI-926 (B). Note different Y-axis scales between the 2 graphs. Identical experiments carried out in HEPM cells treated with either cyclopamine (C) or IPI-926 (D) does not result in Shh hyper-responsiveness. No Shh hyper-responsiveness was observed in C3H10T1/2 cells (E), pancreatic xenograft tumor stroma (F) or SW-872 cells (G) when pre-treated with 5 µM cyclopamine or 500 nM IPI-926.