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. 2014 Mar 7;9(3):e90553. doi: 10.1371/journal.pone.0090553

Figure 2. GP73 increases HCV production in the supernatant.

Figure 2

(A) Schematic of the experimental procedure. Huh7.5.1 cells were infected with the lentivirus carrying the coding sequence of GP73 (pRlenti-GP73), vector control (pRlenti), shRNA expression cassette expressing shRNA against GP73 (shGP73-1 and shGP73-2), or non-target control (shNT) for 6 h. After 24 h, Huh7.5.1 cells were infected with HCV-GFP at 0.02 MOI for another 6 h. The GP73 mRNA levels (B), GP73 protein levels (C), the infection efficiency (D), and MFI (E) of Huh7.5.1 cells were assayed 72 h after infection with HCV-GFP. The HCV-GFP titer (F) and HCV viral RNA levels (G) in the supernatant were assayed by flow cytometry or qRT-PCR. The value of pRlenti-GP73 is represented relative to that of pRlenti. The values of shRNAs against GP73 are represented relative to those of shNT. The results are presented as mean ± SEM derived from three experiments (*P<0.05; **P<0.01).