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. 2014 Mar 7;9(3):e90553. doi: 10.1371/journal.pone.0090553

Figure 4. GP73 increases the secretion of HCV through the coiled-coil domain.

Figure 4

Huh7.5.1 cells infected with HCV-GFP at 0.02 MOI were transfected with GP73 truncation expression plasmids at 48 h post-infection. At 48 h after transfection, Huh7.5.1 cells were harvested and assayed. (A) Protein expression levels of GP73 truncations were detected by western blot. Infection efficiency (B) and MFI (C) of HCV-infected Huh7.5.1 were assayed by flow cytometry (D) Supernatant HCV-GFP titer was assayed by flow cytometry. (E) HCV viral RNA in the supernatant was quantified by qRT-PCR. (F) HCV-GFP titer of the supernatant from Huh7.5.1 cells transfected with indicated plasmids was assayed by flow cytometry. (G) Intracellular HCV-GFP infectivity was assayed by flow cytometry. (H) Intracellular HCV RNA levels were quantified by qRT-PCR. (I) Diagram of GP73 truncation structure and summary of effects on HCV production. (Y: Yes; N: No; UP: Upregulated). The results are presented as mean ± SEM derived from three experiments (*P<0.05; **P<0.01).