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. 2014 Mar 7;9(3):e90715. doi: 10.1371/journal.pone.0090715

Figure 4. SFBLs express significantly higher levels of small leucine-rich proteoglycans.

Figure 4

Relative expression of mRNA for small leucine-rich proteoglycans in GFBL and breast SFBL 3D cultures seven days post-seeding. (A) Expression of mRNA was calculated relative to GFBLs. Results show mean +/− SEM from five parallel cell lines from different donors (*p<0.05, **p<0.01, ***p<0.001; Student's t-test). Range of Ct-values obtained from real-time RT-PCR is indicated below each gene name. (B) Results show relative proportions of mRNA for significantly differently expressed SLRPs in five GFBL and breast SFBL lines from different donors. Decorin was most highly expressed, while fibromodulin was the least abundant in both GFBLs and SFBLs. Expression of total SLRP mRNA was up to 10-fold higher in SFBLs compared to GFBLs. Results were generated by the 2(Ctreference-Cttarget) method. ASPN: Asporin; BGN: Biglycan; DCN: Decorin; FMOD: Fibromodulin; LUM: Lumican.