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. 2014 Mar 7;9(3):e90715. doi: 10.1371/journal.pone.0090715

Figure 5. Expression of MMPs and genes for intracellular ECM turnover is distinct in GFBLs and SFBLs.

Figure 5

Expression of mRNA was analyzed in 3D cultures seven days post-seeding. (A) GFBLs express significantly higher levels of MMP-1, MMP-3, MMP-10 and TIMP-4, and lower levels of MMP-7 and MMP-11 than SFBLs. Expression of mRNA was calculated relative to GFBLs. Results show mean +/− SEM from five parallel cell lines from different donors (*p<0.05, **p<0.01, ***p<0.001; Student's t-test). (B) Results show relative proportions of individual MMPs expressed by the five parallel GFBL and breast SFBL lines. Total MMP expression was up to ten-fold greater in GFBLs compared to SFBLs. In GFBLs, MMP-1, MMP-2 and MMP-10 were the most abundant MMPs, while SFBLs expressed predominantly MMP-2. In general, MMP-7 and MMP-11 were expressed at very low levels compared to other MMPs. Results were generated by the 2(Ctreference-Cttarget) method. (C) SFBLs express significantly higher levels of Endo180 and CTSK than GFBLs. Endo180: also known as CD280; CTSK: Cathepsin K; LRP-1: Low density lipoprotein receptor-related protein-1. A and C: Range of Ct-values obtained from real-time RT-PCR is indicated below each gene name.