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. 2014 Mar 7;9(3):e91003. doi: 10.1371/journal.pone.0091003

Figure 4. Detection of acitretin transport across the BBB.

Figure 4

(A) After 48 hours the amount of acitretin transported across the brain endothelial cell barrier was measured by HPLC. Various concentrations were used to calculate the permeability coefficient of acitretin (Papp (acitretin)). To ensure the tightness of the barrier during the treatment with acitretin, the permeability coefficient of sodium fluorescein was simultaneously determined (acitretin (Papp (NaFITCacitretin)). (B) Induction of ADAM10-promoter activity in SH-SY5Y cells by acitretin transported across endothelial cells. SH-SY5Y cells were transiently transfected with an ADAM10 promoter reporter plasmid and co-cultured with PBECs for 48 hrs. Acitretin was applied to the upper compartment of the transwell system. The induction of ADAM10 promoter activity by acitretin was monitored by measurement of luciferase activity and was normalized to protein content of whole cell lysate. As control, filters without PBEC (w/o PBEC) were used (three experiments; n ≥10; One Way Anova; Bonferroni post-test; ***: p<0.001).