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Acridine orange and ethidium bromide nuclear staining of HLMVECs (A) and HUVECs (B) after 24 hours of DBP-actin treatment. Cells were treated with EGM-2 growth medium, serum started, 1 μM DBP, 1 μM actin or 1 μM DBP-actin complex for 24 hours. Cells were stained with AO/EB and the percentage of live cells was quantified by counting at least 300 cells per treatment. Statistical significance is indicated.
