Figure 3.

Chlorhexidine at 1.0 μg/ml does not increase cell death at 24 h. Photomicrographs used to determine neurite length in Figure 2, were re examined to determine number of cells per high powered field. Dead cells detach from the plate. Therefore a decrease in cell number compared to PLL alone would indicate increased cell death. The average number of cells in each photomicrograph obtained for each condition were counted and averaged for total cell number per high powered field, and divided by average cell number per high powered field in PLL alone to obtain a relative cell number. Four separate experiments were counted and the average relative cell number +/− SD is shown. No significant change in cell number compared to PLL alone was determined.