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. Author manuscript; available in PMC: 2015 Feb 1.
Published in final edited form as: Dev Biol. 2013 Dec 13;386(1):34–41. doi: 10.1016/j.ydbio.2013.12.005

Fig. 5. Changes in expression levels of pro-apoptotic genes is sufficient to predetermine apoptotic outcome.

Fig. 5

(A) qPCR expression analysis shows that mRNA levels of Ark, Nc, and drice (but not dcp-1, dredd and diap1) significantly increase in wL3 animals. qPCR results reflect triplicate biological samples; asterisk indicates p-values <0.01. (B) Western blots show changes in protein expression of Nc and Drice; levels of Diap1 protein, however, are unchanged. β-tubulin was used as loading control. Total mRNA or protein was extracted from appropriately staged eL3 and wL3 whole animals. (C) Effects of reaper expression on animals heterozygous for mutations in Ark82, Nc51 and driceΔ1. All control animals die within hours after hs-rpr (120×) treatment while most triple heterozygous animals survive. Each condition tested in triplicate with at least 20 animals each.