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. Author manuscript; available in PMC: 2015 Feb 1.
Published in final edited form as: Mol Genet Metab. 2013 Sep 21;111(2):172–183. doi: 10.1016/j.ymgme.2013.09.011

Figure 5. Specificity of LC-MS/MS assay to measure psychosine.

Figure 5

(a) A mixture solution of psychosine (432 nM) and glucosyl-psychosine (1,080 nM) standards was prepared in methanol with 0.5% acetic acid and injected to the LC-MS/MS. The BEH Glycan column generated a doublet peak. The early peak corresponds to psychosine and the late peak to glucosylsphingosine. Psychosine and glucosyl-sphingosine are molecular isomers. (b) Lipid extracts from 145M-Twi cells showed a single peak corresponding to psychosine retention time (RT). (c) Lipid extract from the 145C-Wt showed no peak matching either psychosine or glucosyl-sphingosine.