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. Author manuscript; available in PMC: 2015 Feb 1.
Published in final edited form as: Mol Genet Metab. 2013 Sep 21;111(2):172–183. doi: 10.1016/j.ymgme.2013.09.011

Figure 7. Testing throughput LC-MS/MS assay against a known psychosine-reducing agent.

Figure 7

(a) In 96-well plates, the 145M-Twi and 145C-Wt derived from the Twi and respective control mice were cultured and treated for 9 days with L-cycloserine at 50 μM, 100 μM and 250 μM before measuring cellular psychosine levels by LC-MS/MS. (b) Significant reductions of psychosine were observed in 145M-Twi cells with L-cycloserine in cultured medium at various concentrations (n=6). No effect of L-cycloserine was observed in the 145C-Wt (CTRL).