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(A) Immunoblot analysis in denaturing conditions of 293T cells transfected with plasmids expressing the indicated proteins. (B) Lysates from (A) were immunoprecipitated (IP) in denaturing conditions with anti-Flag beads, and the precipitates subsequently analyzed by immunoblotting (IB) with the indicated antibodies. (C) as in (A) and (B) but for HeLa and U2OS cells.
