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. 2014 Mar 7;9(3):e91072. doi: 10.1371/journal.pone.0091072

Figure 2. N-Myc is SUMOylated at lysine 349.

Figure 2

(A) Mouse N-Myc protein sequence (from aa 301 to 450) with consensus SUMO acceptor sites (as predicted by SUMOplot™) highlighted in red. (B) 293T cells were transfected with plasmids expressing the indicated proteins. Lysates were analyzed by immunoprecipitation and immunoblotting as indicated in Fig. 1. (C) The Myc region corresponding to the SUMO acceptor site in mouse N-Myc is aligned with N-Myc sequences from other species and also c-Myc and L-Myc corresponding regions. Mouse N-Myc lysine 349 is highlighted in red.