Skip to main content
. 2014 Mar 7;9(3):e91072. doi: 10.1371/journal.pone.0091072

Figure 6. Protein stresses induce N-Myc SUMOylation.

Figure 6

(A) HeLa cells were transfected with plasmids expressing the indicated proteins and either mock treated or treated for 30 min with 0.7 M NaCl, 3.7% EtOH or heat-shock at 43°C (HS). Lysates were analyzed by immunoprecipitation and immunoblotting in denaturing conditions with the indicated antibodies. The histogram at the bottom represents the quantification of the ratio of monoSUMOylated to total wild-type N-Myc, normalized to the mock-treated cells: values represent the mean and s.d. from five independent experiments. (B) Lan-1 cells were either mock treated, treated with 10 µM MG132 for 6 h or heat-shocked at 43°C for 1 h (HS). Lysates were analyzed as in A. CTRL IP: IP with N-Myc antibody without cell lysate.