(A)Fibroblast-like morphology of GTKO/hCD46 pAdMSC (light microscopy 10×) (B) Adipogenic differentiation of GTKO/hCD46 pAdMSC – Oil red stain (20×). (Insert –fat droplets stained with oil red-40×). (C) Osteogenic differentiation of GTKO/hCD46 pAdMSC – Von Kossa stain (20×), showing morphological changes in pAdMSC and linear calcium deposition (black). (D) Chondrogenic differentiation of GTKO/hCD46 pAdMSC – Alcian blue stain (10×), showing strongly acidic mucosubstance (blue) and goblet cells (red nuclei). (Reproduced from Kumar G, et al, Cytotherapy 2012; 14:494–504 [14], with permission).