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. Author manuscript; available in PMC: 2015 Feb 1.
Published in final edited form as: Stem Cell Rev. 2014 Feb;10(1):79–85. doi: 10.1007/s12015-013-9478-8

Figure 3. Immunogenicity of pMSCs.

Figure 3

(Left) Using serum from two healthy human volunteers (one with high [Human 1] and one with low [Human 2] levels of anti-pig antibodies), IgM/IgG antibody binding to WT and GTKO pMSC was measured by flow cytometry. IgM/IgG binding to GTKO pMSC was less than that to WT pMSC. Relative mean immunoflorescence intensity (MFI) was calculated by dividing the MFI of the tested serum sample by the MFI of the background (secondary antibody binding to target cells). Data are representative of three different experiments.

(Right) Proliferative responses of PBMC from two different healthy humans to WT and GTKO pMSC in mixed lymphocyte reaction (responder:stimulator ratio 1:10). In both cases, the response to GTKO pMSC was weaker than that to WT pMSC (p<0.01 vs. WT pMSC).

(Reproduced from Ezzelarab M, et al. Xenotransplantation 2011; 18:183–195 [13] with permission).