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. Author manuscript; available in PMC: 2015 Feb 1.
Published in final edited form as: Stem Cell Rev. 2014 Feb;10(1):79–85. doi: 10.1007/s12015-013-9478-8

Figure 5. Suppression of human CD4+ and CD8+ T cell proliferation by genetically-modified GTKO/CD46 pMSC.

Figure 5

In MLR, human CD4+ (left) or CD8+ (right) T cell proliferation was measured after coculture with GTKO/CD46 pMSC, or with GTKO pAEC (at a responder:stimulator ratio of 1:10). Also, human T cells were cocultured with GTKO/CD46 pMSC mixed with GTKO pAEC at a 1:1 ratio. GTKO/CD46 pMSC significantly downregulated the responses of CD4+ or CD8+ T cells in response to GTKO pAEC. The proliferation was measured after 5 days of culture, and 16h after addition of 3H-thymidine (1µCi/well) using a beta-scintillation counter (PerkinElmer, Waltham, MA). The results are represented as counts per minute (CPM). Data represent 2 different experiments, and are presented as mean ± SD of triplicates. * p<0.01