Skip to main content
. Author manuscript; available in PMC: 2015 Feb 4.
Published in final edited form as: J Am Coll Cardiol. 2013 Oct 16;63(4):358–368. doi: 10.1016/j.jacc.2013.09.025

Figure 3. Human CYB561 3’-UTR variant A+1485G: Functional studies in cells transfected with a chimeric luciferase / human CYB561 3’-UTR reporter.

Figure 3

Figure 3

Figure 3

Figure 3

Figure 3

3A. Effect of 3’-UTR allele on gene expression in PC12 chromaffin cells. The A-allele displays lower gene expression at each time point (p<0.001).

3B. CYB561 3’-UTR variant A+1485G: Coordinate effects on gene expression in cells as well as HR in humans in vivo. Cellular data are from transfection (Figure 3A), while in vivo data are from human HR in twin pairs (Figure 2). Results are plotted for an A-allele recessive model.

3C. Micro-RNA hsa-miR-1294 mimic and CYB561: Effects on 3’-UTR variant A+1485G in HEK-293T cells. The A-allele, with a better match for the hsa-miR-1294 motif, experiences a more substantial decline of gene expression when co-transfected with the hsa-miR-1294 mimic.

3D. Micro-RNA hsa-miR-1294 mimic and CYB561: Effects on 3’-UTR variant A+1485G in PC12 chromaffin cells. The experimental protocol proceeds as in Figure 3C.

3E. Micro-RNA hsa-miR-1294 inhibition and CYB561: Effects on 3’-UTR variant A+1485G in HEK-293T cells. The A-allele, with a better match for the hsa-miR-1294 motif, experienced a more substantial increase of reporter activity when co-transfected with the hsa-miR-1294 inhibitor.