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. Author manuscript; available in PMC: 2015 Feb 4.
Published in final edited form as: J Am Coll Cardiol. 2013 Oct 16;63(4):358–368. doi: 10.1016/j.jacc.2013.09.025

Figure 4. CYB561 mRNA: Effects of miR-1294.

Figure 4

Figure 4

Figure 4

4A. Micro-RNA effects in HEK-293T cells: hsa-miR-1294 mimicry on human CYB561 mRNA expression with its cognate 3’-UTR. The full-length human CYB561 mRNA, with its cognate 3’-UTR, was expressed under control of a eukaryotic pCMV promoter in HEK-293T cells, with or without co-transfection of a miR-1294 mimic. CYB561 mRNA abundance was quantified by qRT-PCR, and normalized to GAPDH mRNA.

4B. Micro-RNA effects in PC12 chromaffin cells: hsa-miR-1294 mimicry on human CYB561 mRNA expression with its cognate 3’-UTR. The experimental protocol proceeds as in Figure 4A.

4C. Endogenous RNAs in model neuronal/neuroendocrine cells: CYB561 mRNA and miR-1294 small RNA. RNAs were quantified by qRT-PCR, normalizing mRNA results to 18S rRNA and miR results to small RNA SNORD61. The lower limit of detection (LLD) is ~10 molecules per qRT-PCR reaction.