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. Author manuscript; available in PMC: 2015 Feb 1.
Published in final edited form as: Mol Genet Metab. 2013 Oct 19;111(2):184–192. doi: 10.1016/j.ymgme.2013.10.010

Figure 1. Efficient siRNA-mediated silencing of α-gal A in MDCK cells.

Figure 1

MDCK cells were transfected with control siRNA or siRNA targeted against α-gal A. After three days or seven days with repeated transfection, cells were solubilized and mRNA was extracted. (A) The efficiency of knockdown was quantified by RT-PCR, and a representative gel is shown. The migration of DNA ladder standards is shown on the left. The predicted PCR product for α-gal A is 898 bp. β-actin was used as a PCR control (expected product size 160 bp). (B) Quantitation of knockdown efficiency by qRT-PCR, performed as described in Methods. The mean +/− range of two independent experiments is plotted.