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. Author manuscript; available in PMC: 2015 Feb 1.
Published in final edited form as: Mol Genet Metab. 2013 Oct 19;111(2):184–192. doi: 10.1016/j.ymgme.2013.10.010

Figure 3. α-gal A siRNA silencing causes accumulation of zebra bodies and increased lysosome size.

Figure 3

(A) Indirect immunofluorescence of the late endosome/lysosome marker LAMP-2 in control (left panel) and α-gal A silenced (right panel) MDCK cells. SYTOX Green Nucleic Acid Stain was used to visualize nuclei. Scale bar: 10 μm (B) The average area of individual LAMP-2 positive compartments in control and α-gal A silenced cells was quantified using ImageJ. The graph shows data from 20 fields in three independent experiments. *t-test p<0.001. (C) Transmission electron micrographs of MDCK cells treated with α-gal A siRNA for six days (left and middle panels) or six weeks (right panel). Transversely-stacked, osmiophilic myelin-like membranes also known as “zebra bodies” (arrow heads) are evident within six days of transfection and are more prevalent after six weeks of repeated transfections. Scale bar: left: 2 μm, middle and right: 500 nm.