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. Author manuscript; available in PMC: 2015 Feb 21.
Published in final edited form as: Chem Soc Rev. 2013 Aug 22;43(4):1189–1200. doi: 10.1039/c3cs60208g

Figure 4.

Figure 4

a) Experimental design employed by Revyakin et al. to detect single Pol II transcription complexes by colocalizing DNA template molecules with Cy3- and Cy5-labeled floppy probes complementary to the transcript. Immobilized Cy5-DNA templates were imaged, their locations determined, and photobleached prior to addition of the transcription assay components. b) To monitor both transcription and dynamics of the eukaryotic general transcription factors, Revyakin et al. labeled the multicomponent TFIID transcription factor with a quantum dot (QDot 565) and colocalized TFIID fluorescence with DNA templates and Cy5-labeled probes.