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Preparation of fluorescent proteins by fusion to either the SNAP or eDHFR tags. a) Protein-SNAP fusions can be covalently labeled by reaction with benzylguanine dye conjugates. The fluorophore is transferred to an active site cysteine and guanine is released. b) Protein-eDHFR fusions can be non-covalently labeled by incubation with fluorescent derivatives of TMP to form a very tight enzyme-inhibitor complex.
