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. Author manuscript; available in PMC: 2015 Feb 21.
Published in final edited form as: Chem Soc Rev. 2013 Aug 22;43(4):1189–1200. doi: 10.1039/c3cs60208g

Figure 7.

Figure 7

a) Schematic of a CoSMoS experiment designed to study spliceosome assembly. The experiment contains a pre-mRNA fluorescently labeled with Alexa488™ and tethered to a slide surface over which yeast WCE containing Cy5-DHFR-U1 and Dy549-SNAP-U2 is flowed. Spliceosomes then proceed to assemble on the substrate. b) Fluorescence intensity traces generated from the CoSMoS experiment described in 7a. High fluorescence intensity indicates the presence of the fluorescently labeled snRNP. The black and red traces represent the labeled U1 and U2 respectively. The dashed lines tU1 and tU2 mark times at which U1 and U2 associated with the pre-mRNA. This data would indicate that U1 binding precedes U2 association. Data reproduced in this figure from reference 19.