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. Author manuscript; available in PMC: 2015 Feb 20.
Published in final edited form as: J Mol Biol. 2013 Dec 9;426(4):921–935. doi: 10.1016/j.jmb.2013.12.002

Figure 2. Biophysical characterization of wild-type myoc-OLF as a function of pH, in the presence of 200mM NaCl.

Figure 2

(a) Secondary structure of myoc-OLF as a function of pH measured by far-UV CD. (b) Tertiary structure of myoc-OLF as a function of pH measured by near-UV CD. (c) Thermal unfolding of secondary structure monitored by CD at 214 nm. Fit is sigmoidal. (d) Thermal unfolding of tertiary structure monitored by CD at 292 nm. Fit is linear plus sigmoidal. (e) ANS fluorescence as a function of pH. (f) ThT fluorescence as a function of pH at 36 °C monitored for 50 hours. (g) Fibrillar end point morphology for samples incubated at 36 °C in pH 4.6 buffer. (h) Deposits of curvilinear fibrils from samples incubated at 36 °C in pH 3.4 buffer. (i) Disordered aggregates for samples incubated at 36 °C in pH 2.0 buffer. For (g-i), two left panels scale bar = 300nm and two right panels scale bar = 50nm.