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. Author manuscript; available in PMC: 2015 Feb 1.
Published in final edited form as: Mol Cell Biochem. 2013 Nov 6;387(0):177–186. doi: 10.1007/s11010-013-1883-4

Fig. 1.

Fig. 1

m-CN-AGOH inhibits Ras activation. Cells expressing RBDRaf1-GFP and LimE-RFP were treated with m-CN-AGOH (A) or AGOH (B) and stimulated uniformly with 100 μM folic acid. Putative inhibitor m-CN-AGOH (Compound-8, Table 1) inhibited RBDRaf1-GFP and LimE-RFP translocation to membrane. C and T indicate control and treated cells, respectively. Note that treated cells show no Ras activity or actin response. Substrate analog AGOH did not inhibit the translocation of RBDRaf1-GFP or LimE-RFP. Arrow indicates the recruitment of RBDRaf1-GFP and LimE-RFP to the plasma membrane in response to folic acid stimulation (bar, 5 μm)