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. 2013 Oct 28;8(1):142–149. doi: 10.1016/j.molonc.2013.10.007

Figure 5.

Figure 5

Cells with higher percentage of ALDH‐1 more readily for tumor sphere and are resistant to 5‐FU. A. From JHESO, ALDH‐1+ and ALDH‐1− cells are sorted by fluorescence activated cell sorting in 3 cell lines (FLO‐1, JHESO, and OAPC) according to ALDEFLUOR detection kit following the protocol and tumor sphere assays were carried out in triplicate in ultra‐low attachment plate in the tumor sphere medium. After 8–10 days in culture, the number and size of the tumor spheres were counted under a microscope. The ALDH‐1+ cells formed larger tumor spheres and these are numerically shown in the bar graph below. B. The proliferation rate of ALDH1+ and ALDH‐1− cells using the MTS proliferation assay on day 2 and day 6 demonstrate higher proliferative activity of ALDH‐1+ cells. C. ALDH‐1+ cells are more resistant to 5‐FU at different concentrations.