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. Author manuscript; available in PMC: 2015 Jan 22.
Published in final edited form as: Virus Res. 2013 Nov 15;179:231–234. doi: 10.1016/j.virusres.2013.11.007

Fig. 1.

Fig. 1

C1q binds to full recombinant Envelope proteins of all Dengue Virus (DENV1-4) serotypes (A–D, respectively) but not to Bovine Serum Albumin (BSA; negative control). Within each serotype, binding of C1q occurs preferentially to the portion of ENV containing DI/II (A, B and D), except for serotype 3 (C). ELISA plates were coated with DENV ENV full length, ENV DI/II and ENV DIII (aa 1–413, aa 1–296 and aa 297–413, respectively; Table S1) fragments and BSA, and incubated with C1q concentrations ranging from 1 up to 40 µg/ml. Results obtained are representative of 5 independent experiments. Statistical analysis was done using GraphPad program and Student’s paired, 2 tails t-test. Values were considered significant when p<0.05.