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. Author manuscript; available in PMC: 2015 Mar 1.
Published in final edited form as: Ticks Tick Borne Dis. 2013 Nov 16;5(2):127–134. doi: 10.1016/j.ttbdis.2013.09.009

Fig 3.

Fig 3

(A) PCR amplification of spotted fever group Rickettsia spp. in exotic ticks collected from migratory birds. Lane 1: low DNA mass ladder; lanes 2, 4, 6: empty lanes; lane 3: no-template control; lane 5: Rickettsia ompA-positive control; lanes 7–19: rickettsial ompA gene amplified from extracted tick DNA using gene-specific primers in a nested PCR assay. (B) PCR amplification of spotted fever group Rickettsia spp. in migratory songbird blood samples. Lane 1: low DNA mass ladder; lanes 2, 4: empty wells; lane 3: no-template control; lane 5: Rickettsia ompA-positive control, lanes 6–19: rickettsial ompA gene fragment amplified from extract bird blood DNA using gene-specific primers in a nested PCR assay.