Fig. 3.

N^ε-lysine acetylation in peroxisome fractions parallels the induction (proliferation) of peroxisomes in the liver of PPARα-agonist treated animals. Equal amounts (50 µg) of peroxisomal pool fractions proteins isolated from livers of untreated (UT) and ciprofibrate-treated (CIP) animals at different treatment intervals (1, 2, 4, 6, 9, 11, 14 days) were resolved by SDS-electrophoresis, transferred to nitrocellulose and analyzed by western blot using anti-N^ε-acetyl-lysine antibody (a) and antibodies against the PPARα-agonist inducible peroxisomal enzyme Acyl-CoA oxidase-l (ACOX-1; 50 kDa subunit) (b), as described in the “Materials and Methods” . Western blots are representative of at least two individual analyses