Cellular localization of C-terminally tagged genes. Human foreskin fibroblasts (HFFs) were infected with RH expressing hemagglutinin (HA)-tagged TGME49_201860, TGME49_218270 (rop48), TGME49_232020 (ron12) or TGME49_261740 (rop47), fixed and stained with (A) α-HA and rhoptry protein marker α–ROP1, (B) α-HA and dense granule protein marker α-GRA7, α-HA and inner membrane complex marker α-IMC1, α-HA and inner membrane complex marker α-MLP1. (C) α-HA and rhoptry neck/moving junction marker, α-RON4 and (D) α–HA, α-ROP47 and Hoechst dye. (A–C) Scale bars represent 5 µm. (D) Scale bars represent 20 µm.