Representative examples of NY-ESO-1 expression in paired primary and metastatic lesions from the same patient. A, Primary cutaneous melanoma (H&E). B, Negative NY-ESO-1 expression in primary tumor (NY-ESO-1). C, Metastasis in subcutaneous tissue (H&E), D, Expression of NY-ESO-1 in metastasis in subcutaneous tissue. E, Metastasis in colon (H&E). F, Expression of NY-ESO-1 in metastasis in colon. A, C and E, ×4; B, D and F, ×10.