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. Author manuscript; available in PMC: 2015 Feb 1.
Published in final edited form as: Acta Neuropathol. 2013 Oct 18;127(2):243–256. doi: 10.1007/s00401-013-1175-9

Fig. 1. I2PP2A is cleaved into I2NTF and I2CTF and PP2A activity is compromised in spinal cords of sporadic ALS cases.

Fig. 1

A, C) Western blots developed with mAb to I2NTF and pAb to I2CTF show the cleavage of I2PP2A in spinal cords of ten cases of sporadic ALS and three age-matched controls. B, D) Quantitative analysis of the blots shows that the cleavage of I2PP2A into I2NTF and I2CTF (ratio of I2NTF or I2CTF to I2PP2A full-length) is markedly increased in sporadic ALS cases as compared with control group. E, F) PP2A activity determined by the phosphatase ELISA assay is markedly decreased in ALS cases compared with control group. G, H) Western blots developed with anti-PP2Ac mouse monoclonal antibody showed no significant change in the levels of the phosphatase in ALS cases when compared with control group I) β-actin as a loading control. J) PP2A activity inversely correlated to the level of I2CTF in the spinal cord. I2FL = I2PP2A full-length.