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. Author manuscript; available in PMC: 2015 Feb 1.
Published in final edited form as: Acta Neuropathol. 2013 Oct 18;127(2):243–256. doi: 10.1007/s00401-013-1175-9

Fig. 5. I2CTF expression induces hyperphosphorylation and accumulation of neurofilaments and tau, increase in the expression of ubiquitin, and aggregation and translocation of TD43 from the motor neuronal nucleus to the cytoplasm in I2CTF rats.

Fig. 5

a–f: Immunohistochemical staining with anti-non-phosphorylated (np) NF-H mAb (SMI33) showed a decrease in the density of npNF-H-positive motor neurons in the spinal cords of I2CTF rats; g–l: Immunohistochemical staining with anti-phospho (p) NF-H mAb (SMI34) showed an increase in phosphorylation and accumulation of neurofilaments in the axonal tracts of the spinal cords of I2CTF rats; m–r: Immunohistochemical staining with anti-pSer262/356 tau (mAb 12E8) showed abnormal hyperphosphorylation and aggregation of tau in the motor neuron cell cytoplasm reminiscent of stage 0 (r) and stage 1 (p) neurofibrillary tangles, and in dystrophic neurites resembling neuritic plaques (n); s–t: An increase in ubiquitin immunostaining was evident in the axonal tracts in the spinal cords of I2CTF rats; and u–v: Immunohistochemical staining showed condensation and translocation of TDP43 from the motor neuron nucleus to the cytoplasm in the spinal cords of I2CTF rats. a–v: Data from 14-month-old rats. Scale bar: 25 μm.