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. Author manuscript; available in PMC: 2015 Feb 1.
Published in final edited form as: Exp Neurol. 2013 Nov 15;252:1–11. doi: 10.1016/j.expneurol.2013.11.007

Figure 7. Selective SCG10 labeling reveals slowed axon regeneration in cyt-NMNAT1 mice.

Figure 7

(A) Distribution of SCG10 and GAP43 are compared at 1 d after crush in cyt-NMNAT1 mice, a model of delayed Wallerian degeneration. The distal axons are devoid of SCG10 despite of the slowed axon degeneration program. GAP43 robustly stains the distal axons. Scale bar = 500 μm.

(B) At 3 d after crush, the sciatic nerve of cyt-NMNAT1 is immunostained for SCG10, GAP43 and β3 tubulin. SCG10 staining shows that neurons from cyt-NMNAT1 expressing mice display slowed axon regeneration (quantified in (C)). GAP43 labeling persists in the distal axon segments. β3 tubulin shows that the distal cyt-NMNAT1 axons are intact at 3 d after injury. Scale bar = 500 μm.

(C) Axon regeneration in cyt-NMNAT1 mice are quantified by regeneration indices obtained from SCG10 immunostaining at 3 d after crush injury. n = 3 for each condition; ***p < 0.001 by t test. Error bars represent SEM.