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. Author manuscript; available in PMC: 2015 Jan 17.
Published in final edited form as: ACS Chem Biol. 2013 Nov 5;9(1):266–275. doi: 10.1021/cb4006408

Figure 2. Hypoxia induced TG2 activity in mice.

Figure 2

C57BL/6 mice were placed in normobaric hypoxic (FIO2 10.5%) chambers for 5–16 days or left in atmosphere as the normoxia-exposed animal controls. TG2 activity in the lung was measured using the nucleophilic substrate, 5BP, as described in the text. To demonstrate that the observed 5BP incorporation was due to TG2 activity, the TG2 inhibitor, ERW1041E, was contemporaneously administered in animals exposed to 16 days of hypoxia. Alexa Fluor 488 (green) was used to visualize TG2 protein, whereas Alexa Fluor 555 (red) corresponded to TG2 activity. (A) Hypoxia induced TG2 activation in lung tissue compared to normoxic treatments. White scale bars are 100[m in length. (B) Quantitation of specific TG2 activity using ImageJ. At least 30 images per condition were collected of which representative images are shown. Data are represented as average +/− standard error. All images were processed identically.