Table 1.
Polymorphism | Primer sequence (5′-3′) | Tm (°C) | Product size | Restriction enzyme | Allele size | Reference |
---|---|---|---|---|---|---|
Ala307Thr | F: 5′-CCTGCACAAAGACAGTGATG-3′; | 55 | 577 | AhdI | Ala:403 + 174 | [19] |
R: 5′-TGGCAAAGACAGTGAAAAG-3′ | Thr:403 + 143 + 31 | |||||
Ser680Asn | F: 5′-TTTGTGGTCATCTGTGGCTGC-3′; | 60 | 520 | BsrI | Asn:520 | [20] |
R:5′-CAAAGGCAAGGACTGAATTATCATT-3′ | Ser:413 + 107 |
F forward primer, R reverse primer, Tm annealing temperatures, R reference