. 2014 Jan 5;31(3):371–377. doi: 10.1007/s10815-013-0166-z

Table 1.

Primers, annealing temperatures, product sizes, restriction enzymes, and allele sizes

Polymorphism	Primer sequence (5′-3′)	Tm (°C)	Product size	Restriction enzyme	Allele size	Reference
Ala307Thr	F: 5′-CCTGCACAAAGACAGTGATG-3′;	55	577	AhdI	Ala:403 + 174	[19]
Ala307Thr	R: 5′-TGGCAAAGACAGTGAAAAG-3′	55	577	AhdI	Thr:403 + 143 + 31	[19]
Ser680Asn	F: 5′-TTTGTGGTCATCTGTGGCTGC-3′;	60	520	BsrI	Asn:520	[20]
Ser680Asn	R:5′-CAAAGGCAAGGACTGAATTATCATT-3′	60	520	BsrI	Ser:413 + 107	[20]

F forward primer, R reverse primer, Tm annealing temperatures, R reference