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. 2014 Jan 11;31(3):295–306. doi: 10.1007/s10815-013-0152-5

Table 3.

Effect of graded doses of recombinant GDF9 during IVM on subsequent embryo development

Treatment GDF9 (ng/ml) Number of oocytes Cleavagea Blastocyst on day 6b Hatching blastocyst on day 6c ICMd TEd TCNd
Control 0 152 82.9 ± 3.3 81.7 ± 4.4 62.6 ± 8.6 16.0 ± 0.8 53.0 ± 2.4 69.0 ± 2.8
GDF9 50 118 74.6 ± 5.2 72.6 ± 8.9 61.8 ± 8.9 16.8 ± 1.1 54.2 ± 3.6 71.0 ± 4.4
GDF9 100 118 74.8 ± 9.6 82.6 ± 7.8 59.1 ± 7.6 16.7 ± 0.9 49.2 ± 2.6 65.9 ± 3.3
GDF9 200 147 86.1 ± 4.7 82.5 ± 8.3 64.3 ± 7.1 17.9 ± 0.7 56.8 ± 3.5 74.8 ± 3.9

Data are presented as means ± SEM of five replicate experiments. Each replicate experiment consisted of 10–50 oocytes

aPercentage of cleaved embryos per total oocytes

bPercentage of blastocysts generated 100–125 h post-fertilization per cleaved embryo

cPercentage of hatching blastocysts generated 100–125 h post fertilization per cleaved embryo

dMean blastocyst cell numbers following differential staining of 28–51 blastocysts