Table 3.

Effect of graded doses of recombinant GDF9 during IVM on subsequent embryo development

Treatment	GDF9 (ng/ml)	Number of oocytes	Cleavagea	Blastocyst on day 6b	Hatching blastocyst on day 6c	ICMd	TEd	TCNd
Control	0	152	82.9 ± 3.3	81.7 ± 4.4	62.6 ± 8.6	16.0 ± 0.8	53.0 ± 2.4	69.0 ± 2.8
GDF9	50	118	74.6 ± 5.2	72.6 ± 8.9	61.8 ± 8.9	16.8 ± 1.1	54.2 ± 3.6	71.0 ± 4.4
GDF9	100	118	74.8 ± 9.6	82.6 ± 7.8	59.1 ± 7.6	16.7 ± 0.9	49.2 ± 2.6	65.9 ± 3.3
GDF9	200	147	86.1 ± 4.7	82.5 ± 8.3	64.3 ± 7.1	17.9 ± 0.7	56.8 ± 3.5	74.8 ± 3.9

Data are presented as means ± SEM of five replicate experiments. Each replicate experiment consisted of 10–50 oocytes

^aPercentage of cleaved embryos per total oocytes

^bPercentage of blastocysts generated 100–125 h post-fertilization per cleaved embryo

^cPercentage of hatching blastocysts generated 100–125 h post fertilization per cleaved embryo

^dMean blastocyst cell numbers following differential staining of 28–51 blastocysts