Table 5.

Effect of graded doses of GDF9 and BMP15 during IVM on subsequent embryo development

Treatment	GDF9 + BMP15 (ng/ml)	Number of oocytes	Cleavagea	Blastocyst on day 6b	Hatching blastocyst on day 6c	ICMd	TEd	TCNd
Control	0 + 0	78	92.0 ± 1.3	85.4 ± 5.7	84.2 ± 6.9	12.9 ± 0.8	42.8 ± 1.7	55.7 ± 1.9
BMP15 + GDF9	50 + 50	88	91.5 ± 2.7	82.6 ± 9.5	80.5 ± 11.5	12.7 ± 0.7	43.5 ± 2.6	56.2 ± 2.9
BMP15 + GDF9	100 + 100	86	93.2 ± 0.4	78.6 ± 10.8	75.2 ± 11.3	12.9 ± 1.0	37.9 ± 2.1	50.8 ± 2.1
BMP15 + GDF9	200 + 200	85	89.7 ± 5.2	72.3 ± 7.1	67.0 ± 7.6	11.8 ± 1.0	45.5 ± 2.1	57.3 ± 2.7

Data are presented as means ± SEM of three replicate experiments. Each replicate experiment consisted of 12–40 oocytes

^aPercentage of cleaved embryos per total oocytes

^bPercentage of blastocysts generated 100–125 h post-fertilization per cleaved embryo

^cPercentage of hatching blastocysts generated 100–125 h post fertilization per cleaved embryo

^dNumber of blastocysts staining in each treatment group 14–19