Table 6.
Treatments | Pre-treatment of OSFs1 | Number of oocytes | Cleavage2 | Blastocyst on day 53 | Blastocyst on day 64 | Hatching blastocyst on day 65 | ICM6 | TE6 | TCN6 |
---|---|---|---|---|---|---|---|---|---|
Control | N/A | 152 | 87.5 ± 1.6a | 52.2 ± 7.2 | 67.4 ± 6.0a | 53.3 ± 5.6a | 18.7 ± 1.9a | 51.1 ± 3.4 | 69.8 ± 4.3 |
COC + DO | 0h [GV] | 155 | 95.5 ± 2.2b | 57.5 ± 2.6 | 77.2 ± 4.4a,b | 66.9 ± 2.8a,b | 22.0 ± 1.7a,b | 52.4 ± 2.4 | 74.4 ± 3.5 |
COC + DO | 3h + FSH + CCs | 153 | 94.8 ± 1.1a,b | 65.5 ± 1.7 | 83.4 ± 2.6b | 74.5 ± 2.8b | 24.8 ± 1.3b | 55.7 ± 2.7 | 80.5 ± 3.4 |
Values with different superscripts within a column are statistically different (P < 0.05)
Data are presented as means ± SEM of four replicate experiments. Each replicate experiment consisted of 37–40 oocytes
1See Fig. 1 and Table 1 for explanation of experimental design
2Percentage of cleaved embryos per total oocytes
3Percentage of blastocysts generated 96–100 h post-fertilization per cleaved embryo
4Percentage of blastocysts generated 100–125 h post-fertilization per cleaved embryo
5Percentage of hatching blastocysts generated 100–125 h post fertilization per cleaved embryo
6Mean blastocyst cell numbers following differential staining of 13–22 blastocysts