Table 6.

Effects of exogenous native OSFs during IVM on subsequent embryo development

Treatments	Pre-treatment of OSFs1	Number of oocytes	Cleavage2	Blastocyst on day 53	Blastocyst on day 64	Hatching blastocyst on day 65	ICM6	TE6	TCN6
Control	N/A	152	87.5 ± 1.6a	52.2 ± 7.2	67.4 ± 6.0a	53.3 ± 5.6a	18.7 ± 1.9a	51.1 ± 3.4	69.8 ± 4.3
COC + DO	0h [GV]	155	95.5 ± 2.2b	57.5 ± 2.6	77.2 ± 4.4a,b	66.9 ± 2.8a,b	22.0 ± 1.7a,b	52.4 ± 2.4	74.4 ± 3.5
COC + DO	3h + FSH + CCs	153	94.8 ± 1.1a,b	65.5 ± 1.7	83.4 ± 2.6b	74.5 ± 2.8b	24.8 ± 1.3b	55.7 ± 2.7	80.5 ± 3.4

Values with different superscripts within a column are statistically different (P < 0.05)

Data are presented as means ± SEM of four replicate experiments. Each replicate experiment consisted of 37–40 oocytes

¹See Fig. 1 and Table 1 for explanation of experimental design

²Percentage of cleaved embryos per total oocytes

³Percentage of blastocysts generated 96–100 h post-fertilization per cleaved embryo

⁴Percentage of blastocysts generated 100–125 h post-fertilization per cleaved embryo

⁵Percentage of hatching blastocysts generated 100–125 h post fertilization per cleaved embryo

⁶Mean blastocyst cell numbers following differential staining of 13–22 blastocysts