Table 1.
MicroRNA validation raw cycle threshold (Ct) and fold change relative to U6snRNA (control probe) using RT-PCR
miRNA | Raw Ct PCOS |
Raw Ct OD |
Fold change |
---|---|---|---|
U6snRNA | 20.1 | 20.1 | 1 |
miR-32 | 28.0 | 30.9 | 7.5* |
miR-34c | 26.3 | 32.2 | 59.7* |
miR-135a | 28.2 | 30.7 | 5.7* |
miR-138 | 29.5 | 32.1 | 6.1 |
miR-142 | 32.7 | 35.6 | 7.5 |
miR-18b | 27.5 | 30.0 | 5.7* |
miR-489 | 20.4 | 21.6 | 2.3 |
miR-627 | 16.8 | 18.6 | 3.5 |
miR-888 | 24.6 | 27.3 | 6.5 |
miR-9 | 31.8 | 38.5 | 104.0* |
* p < 0.05