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. Author manuscript; available in PMC: 2015 Feb 1.
Published in final edited form as: Mol Imaging Biol. 2014 Feb;16(1):74–84. doi: 10.1007/s11307-013-0674-3

Fig. 7.

Fig. 7

Fluorescence microscopy of mouse brains with implanted U87-Luc or U251-Luc tumors excised after imaging studies. Mice bearing a U251-Luc or U87-Luc tumor were injected intravenously with Cy5.5-TNYL-RAWK-64Cu-DOTA peptides. Signal from Cy5.5 is pseudocolored purple. EphB4 (green) was stained with rabbit anti-human EphB4 antibody and Alexa Fluor 488-conjugated goat anti-rabbit immunoglobulin. CD31 (red), which is the marker of the endothelial cells of tumor-associated blood vessels, was stained with rat anti-mouse CD31 antibody and Alexa Fluor 594-conjugated donkey anti-rat antibody. Cell nuclei were counterstained with DAPI (blue). Bar=40 μm.