Table 2.

CPY-Invertase secretion assay.

Construct	% CPY-Invertase secretion (normalized values, average of three experiments)
Empty vector	0 ± 0.4
Vps4(E233Q)	100 ± 4.8
GFP-FYVE	6 ± 0.2
MIT-GFP-FYVE	6 ± 0.2
MIT(L64D)-GFP-FYVE	10 ± 0.3
MIT(I18D)-GFP-FYVE	10 ± 0.3
MIT(L64D,I18D)-GFP-FYVE	9 ± 0.3
Bro1(N)-GFP-FYVE	−6 ± 0.4
GFP-Doa4(C)-FYVE	−3 ± 0.6
GFP-Doa4(C,AAFA)-FYVE	−6 ± 0.4
Did2(N)-GFP-FYVE	−3 ± 0.5
2Ub-GFP-FYVE	18 ± 0.9
Vps27(PSDP)-GFP-FYVE	−2 ± 0.5
Vps28(C)-GFP-FYVE	−7 ± 0.2