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. Author manuscript; available in PMC: 2015 Mar 1.
Published in final edited form as: J Comp Neurol. 2014 Mar;522(4):876–899. doi: 10.1002/cne.23451

Figure 11. Rax heterozygotes show increased detyrosinated α tubulin-positive cilia in the α2 tanycytic zone.

Figure 11

(A, B) Confocal optical slides of immunohistochemistry for vimentin (Vim) immunohistochemistry (magenta) and detyrosinated α tubulin (G-TUB) (green). G-TUB+ Primary cilia are more abundant in the α2 tanycytic zone of Rax+/− mice (white arrow heads in B), compared to Rax+/+ mice (A). (C) Quantification of multiple cilia clusters in each optical slice of z-stack confocal pictures at three different Bregma points. The cumulative total indicates the sum of multiple cilia along the three different Bregma points (from −1.555 mm to −1.755 mm). Unpaired t-test Rax+/+ (n=4), Rax+/− (n=3). Bregma −1.655 mm p= 0.001, Bregma −1.755 mm p=0.02, cumulative p=0.04. Scale bar: 20 μm.