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. Author manuscript; available in PMC: 2015 Mar 1.
Published in final edited form as: J Comp Neurol. 2014 Mar;522(4):876–899. doi: 10.1002/cne.23451

Figure 2. Rax is co-expressed with vimentin in the ventral portion of the third ventricle wall.

Figure 2

Confocal z-stack reconstruction of Rax fluorescent in situ hybridization (fISH) (magenta) and vimentin (Vim) immunohistochemistry (green) counterstained with DAPI in brain sections of adult C57BL/6 wild type mice (P45). (A–T) Rax is expressed along the anteroposterior axis of the ventral hypothalamus and co-localizes with vimentin in the ventral portion of the wall of the third ventricle (white arrow heads). Numbers in left bottom corner correspond to different Bregma points. Bregma −1.555mm (A–D) represents the anterior hypothalamus, Bregmas −1.655mm to −1.955 mm (E–P) represent the medial hypothalamus and Bregma −2.055mm (Q–T) represents the posterior hypothalamus. Scale bar: 100μm. 3V: third ventricle.