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. Author manuscript; available in PMC: 2015 Mar 1.
Published in final edited form as: J Comp Neurol. 2014 Mar;522(4):876–899. doi: 10.1002/cne.23451

Figure 4. Rax and Gpr50 are expressed within the median eminence.

Figure 4

Orthogonal view of Rax (green) and Gpr50 (magenta) double fISH combined with vimentin (Vim) immunohistochemistry (white) counterstained with DAPI (blue) in the median eminence of adult C57BL/6 wild type mice (P45) (A) Rax and Gpr50 mRNAs are not only expressed in β tanycytes located in the ependymal layer (EL) of the ME (white arrow heads), but also in other layers of the ME in close association with tanycyte processes (yellow arrows). This localization suggests that Rax and Gpr50 are translated in tanycyte processes. (B–K) Orthogonal view of the dashed square area showing a Rax+, Gpr50+ nucleus in close association with Vim staining. These Rax+, Gpr50+ cells might correspond to “astrocytic tanycytes” EL, Ependymal layer, SE, subependymal layer, FL, fiber layer, RL, reticular layer, PL, palisade layer. 3V: third ventricle. Scale bar: 20 μm.