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. Author manuscript; available in PMC: 2015 Mar 1.
Published in final edited form as: J Comp Neurol. 2014 Mar;522(4):876–899. doi: 10.1002/cne.23451

Figure 7. Rax mRNA is reduced in Rax heterozygote mice.

Figure 7

Confocal z-stack reconstruction of Rax fISH (magenta) and vimentin (Vim) immunohistochemistry (green) counterstained with DAPI (blue) in the α2 tanycytic zone of the medial hypothalamus (Bregma −1.655mm). Rax mRNA signal is reduced in Rax heterozygotes mice (Rax+/−) (E) compared to Rax wild type mice (Rax+/+) (A). (I) Digital quantification of Rax fISH signal in the α2 tanycytic zone using a three dimensional reconstruction of the signal with the spot tool of the Imaris software Unpaired t-test (n=3), p=0.01. 3V: third ventricle. Scale bar 20μm.