Table 1.

SPG4-derived neurons have decreased frequency of retrograde fast axonal transport motile events.

	Anterograde Motile Velocity (μm/s)	Retrograde Motile Velocity (μm/s)	Events (>300 nm/s)	Anterograde Events (>300 nm/s)	Retrograde Events (>300 nm/s)
iWT-e3	0.48±0.21	0.50±0.19	0.43±0.09	0.15±0.04	0.29±0.07
iSPG4-e6	0.45±0.12	0.54±0.17	0.14±0.04	0.08±0.02	0.06±0.02
P-value	ns	ns	<0.01	ns	<0.01

The velocity and frequency of mitochondrial motility were quantified in week 8 control and SPG4-derived neurons. No velocity differences were found in either the anterograde or retrograde direction. The frequency of total motile events was significantly decreased in SPG4 neurons. Though there was a non-significant trend toward reduction of anterograde motile events in SPG4 neurons, retrograde events were significantly reduced. Data are presented as means ±SEM. Statistical significance was determined using a two-sided t-test.