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. Author manuscript; available in PMC: 2015 Apr 1.
Published in final edited form as: Int J Cancer. 2013 Oct 15;134(7):1758–1766. doi: 10.1002/ijc.28499

Fig. 3. In vitro pro-apoptotic effects of Dox and MN-siBIRC5 in pancreatic adenocarcinoma (CAPAN-2) cells.

Fig. 3

(A) Effect of the combined drugs on caspase-3 activation. Cells were treated with PBS and Dox (0.1μM), either alone or in sequential combination with MN-siBIRC5 or MN-siSCR. For the sequential combination treatments, Dox was either added for 24h prior to MN-siBIRC5/MN-siSCR or in the reverse sequence. Caspase-3 activity was measured in cell lysates after 48h incubation. Caspase-3 activation was significantly higher in the Dox-MN-siBIRC5 treatment group relative to PBS. Data expressed as mean ± SD and are representative of three independent experiments (n=3; two-tailed Student t-test; ***P<0.0005). (B) Analysis of PARP cleavage. After exposing the cells to drug treatments (as above), immunofluorescence was done to detect cleaved PARP fragments. Nuclei (DAPI, Blue) and cleaved PARP 29 were visualized by fluorescence microscopy. PARP cleavage was most prominent in the Dox-MN-siBIRC5 treatment group.